Nucleic acid constructs and methods of use

摘要

The present invention provides oligonucleotide constructs, sets of such oligonucleotide constructs, and methods of using such oligonucleotide constructs to provide validated sequences or sets of validated sequences corresponding to desired ROIs. Such validated ROIs and constructs containing these have a wide variety of uses, including in synthetic biology, quantitative nucleic acid analysis, polymorphism and/or mutation screening, and the like.

主权项

1. A method for determining the sequences of a set of nucleic acid targets, comprising:
tagging each individual nucleic acid target in a set of nucleic acid targets to create a set of tagged constructs, with: (a) at least one unique identifier oligonucleotide that distinguishes the tagged construct from other tagged constructs in the set, wherein the at least one unique identifier oligonucleotide is not part of the nucleic acid target; and (b) at least one amplification site; amplifying the set of tagged constructs; and determining the sequences of the amplified set of tagged constructs comprising the unique identifier oligonucleotides and the nucleic acid targets.