L-lactate production in cyanobacteria

摘要

A process of producing L-lactate by feeding carbon dioxide to a culture of a cyanobacterial cell and subjecting this culture to light, wherein the cell is capable of expressing a nucleic acid molecule, which upon expression confers on the cell the ability to convert a glycolytic intermediate into L-lactate and wherein aforementioned nucleic acid molecule is under the control of a regulatory system which responds to light or to a change in the concentration of a nutrient in the culture.

主权项

1. A process of producing L-lactate by feeding carbon dioxide to a culture of a cyanobacterial cell and subjecting said culture to light, wherein said cell comprises a nucleic acid molecule encoding an L-lactate dehydrogenase that is not sensitive towards oxygen inactivation, and wherein the expression of said nucleic acid molecule confers on the cell the ability to convert a glycolytic intermediate into L-lactate, wherein the nucleic acid molecule comprises a nucleotide sequence encoding a L-lactate dehydrogenase, and wherein said nucleotide sequence is selected from the group consisting of:
i. nucleotide sequences encoding a L-lactate dehydrogenase, said L-lactate dehydrogenase comprising an amino acid sequence that has at least 50% sequence identity with the amino acid sequence of SEQ ID NO:2; ii. nucleotide sequences comprising a nucleotide sequence that has at least 50% sequence identity with the nucleotide sequence of SEQ ID NO:1; iii. nucleotide sequences the reverse complementary strand of which hybridizes to a nucleic acid molecule of a sequence of (i) or (ii); and iv. nucleotide sequences the sequences of which differ from the sequence of a nucleic acid molecule of (iii) due to the degeneracy of the genetic code.