Reference control for cell by cell analysis

摘要

A reference control for cell by cell analysis on flow cytometric analyzers contains cellular analogs made of permeated blood cells containing therein aggregated intracellular proteins and preserved antigenic sites thereof, having cellular membrane permeable to antibodies and a suspension medium. The reference control is frozen after being prepared and thawed prior to use. The cellular analogs further contain a fluorescence marker therein. Further disclosed are a method of making the reference control and a method using the reference control, as an internal or stand-alone control, for measurements of cellular hemoglobin and cellular hemoglobin variant of a blood sample.

主权项

1. A reference control for cell by cell analysis on a flow cytometric analyzer, comprising
cellular analogs, wherein said cellular analogs comprise permeated blood cells containing therein aggregated intracellular proteins and one or more preserved antigenic sites thereof, and having cellular membrane permeable to an antibody; and a suspensionstorage medium comprising a saccharide, wherein said suspensionstorage medium does not contain glycerol or dimethylsulfoxide, said reference control is freeze-thaw stable and can undergo one or more cycles of freezing and thawing, wherein said cellular analogs maintain said one or more preserved antigenic sites of said proteins after being thawedwherein said cellular analogs are suspended in said storage medium and said cellular analogs maintain said one or more preserved antigenic sites of said proteins after freezing and thawing said reference control.