METHODS AND MEANS FOR THE PRODUCTION OF IG-LIKE MOLECULES

摘要

The invention provides means and methods for producing one or more Ig-like molecules in a single host cell. Novel CH3 mutations enabling the production of monospecific and/or bispecific Ig-like molecules of interest are also provided.

主权项

1. A method for producing at least two different Ig-like molecules from a single host cell, wherein each of said two Ig-like molecules comprises two CH3 domains that are capable of forming an interface, said method comprising providing in said cell
a. A first nucleic acid molecule encoding a 1st CH3 domain-comprising polypeptide chain, b. A second nucleic acid molecule encoding a 2nd CH3 domain-comprising polypeptide chain, c. A third nucleic acid molecule encoding a 3rd CH3 domain-comprising polypeptide chain, and d. A fourth nucleic acid molecule encoding a 4th CH3 domain-comprising polypeptide chain,wherein at least two of said nucleic acid molecules are provided with means for preferential pairing of said 1st and 2nd CH3 domain-comprising polypeptides and said 3rd and 4th CH3-domain comprising polypeptides, said method further comprising the step of culturing said host cell and allowing for expression of said at least four nucleic acid molecules and harvesting said at least two different Ig-like molecules from the culture.