Method for quantifying the amount of cholesterol in high-density lipoprotein 3

摘要

Disclosed is a method for quantifying the amount of cholesterol in high-density lipoprotein 3 (HDL3) in a test sample without requiring cumbersome procedures. Said method includes: a first step in which a test sample is reacted with a surfactant that reacts with lipoproteins other than high-density lipoprotein 3 and cholesterol is moved outside of the reaction system; and a second step in which the amount of cholesterol remaining in the reaction system is quantified. This makes it possible to use an automated analyzer to specifically quantify the amount of HDL3 cholesterol in a test sample without requiring cumbersome work such as ultracentrifuging and pretreatment. It is also possible to quantify the amount of HDL2 cholesterol by subtracting out the HDL3 cholesterol amount from a total HDL cholesterol amount obtained via an existing method for quantifying the total HDL cholesterol in a sample.