| 摘要 |
The observation device 1 is a device capable of substantially simultaneously acquiring the fluorescence image and the interference image on the common observation plane in the observation object 90 mounted on slide glass 41, and has an excitation light source 11, a filter 12, a dichroic mirror 13, a lens 14, a dichroic mirror 15, an objective lens 16, a filter 17, a fluorescence imaging unit 18, an interference imaging light source 21, a filter 22, a dichroic mirror 23, a lens 24, a half mirror 25, an objective lens 26, a lens 27, a dichroic mirror 28, an interference imaging unit 29, a position detection light source 31, a light detection unit 32, the slide glass 41, an actuator 42, a reference mirror 43, an actuator 44, a control unit 51, and a display unit 52. |
| 主权项 |
1. An observation device comprising:
an excitation light source for outputting excitation light; an objective lens for implementing irradiation of an observation object with the excitation light output from the excitation light source and receiving fluorescence generated in the observation object in accordance with the irradiation; a fluorescence imaging unit for taking an image of the fluorescence generated in the observation object and guided through the objective lens; a mount unit for the observation object to be mounted thereon, which is movable in an optical-axis direction of the objective lens; an interference imaging light source for outputting interference imaging light; a position detection light source for outputting position detection light; a multiplexing unit for multiplexing the interference imaging light output from the interference imaging light source and the position detection light output from the position detection light source and outputting the light multiplexed; an interference optical system for splitting the light multiplexed and output by the multiplexing unit, into two beams and outputting the two beams as a first branch beam and a second branch beam, for implementing application of the first branch beam through the objective lens onto the observation object or onto the mount unit and receiving input of first reflection generated in accordance with the application, for implementing application of the second branch beam onto a reference mirror and receiving input of second reflection generated in accordance with the application, and for letting the first reflection and the second reflection interfere with each other and outputting resultant light; a demultiplexing unit for demultiplexing the light output from the interference optical system into the interference imaging light and the position detection light and outputting the interference imaging light and the position detection light; an interference imaging unit for taking an interference image of the interference imaging light output from the demultiplexing unit; a light detection unit for implementing detection of interference intensity of the position detection light output from the demultiplexing unit; and a control unit for acquiring the result of the detection by the light detection unit and for controlling a positioning operation of the mount unit, an imaging operation by the fluorescence imaging unit, and an imaging operation by the interference imaging unit, wherein the control unit performs as follows: the control unit determines an optical path length difference between an optical path of the position detection light from the position detection light source via the mount unit to the light detection unit and an optical path of the position detection light from the position detection light source via the reference mirror to the light detection unit, based on the result of the detection of the interference intensity by the light detection unit; the control unit performs feedback control based on the optical path length difference thus determined, thereby to set a position of the mount unit to a target position; the control unit makes both or either one of the imaging operation by the fluorescence imaging unit and the imaging operation by the interference imaging unit carried out at the target position of the mount unit. |
