RIFT VALLEY FEVER VIRUS REPLICON PARTICLES AND USE THEREOF

摘要

Disclosed herein is a robust system for the reverse genetics generation of a Rift Valley fever (RVF) virus replicon particle (VRPRVF) vaccine candidate. VRPRVF can actively synthesize viral RNA and proteins, but lack structural glycoprotein genes, preventing spread within immunized individuals and reducing the risk of vaccine-induced pathogenicity. Is it disclosed herein that VRPRVF immunization is both safe and efficacious, resulting in a robust immune response that is protective against RVF virus challenge within 24 hours of immunization. Provided herein are VRPRVF, methods of producing VRPRVF, and method of using VRPRVF for immunization against RVF virus infection.

主权项

1. A Rift Valley fever (RVF) virus replicon particle (VRPRVF), comprising:
(i) RVF virus Gn and Gc glycoproteins; (ii) RVF virus polymerase protein; (iii) RVF virus nucleoprotein; (iv) an RVF virus L genome segment; and (v) an RVF virus S genome segment,wherein the VRPRVF does not contain an RVF virus M segment or any nucleic acid molecule encoding the Gn and Gc glycoproteins.