发明名称 |
Novel Structurally Designed shRNAs |
摘要 |
Provided is an improved design of shRNA based on structural mimics of miR-451 precursors. These miR-451 shRNA mimics are channeled through a novel small RNA biogenesis pathway, require AGO2 catalysis and are processed by Drosha but are independent of DICER processing. This miRNA pathway feeds active elements only into Ago2 because of its unique catalytic activity. These data demonstrate that this newly identified small RNA biogenesis pathway can be exploited in vivo to produce active molecules. |
申请公布号 |
US2015197749(A1) |
申请公布日期 |
2015.07.16 |
申请号 |
US201514630419 |
申请日期 |
2015.02.24 |
申请人 |
Hannon Gregory J.;Cheloufi Sihem |
发明人 |
Hannon Gregory J.;Cheloufi Sihem |
分类号 |
C12N15/113 |
主分类号 |
C12N15/113 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
Cold Spring Harbor NY US |