HUMAN TISSUE KALLIKREIN 1 GLYCOSYLATION ISOFORMS

摘要

Provided are preparations of tissue kallikrein-1 (KLK1) glycoforms having a defined number of oligosaccharide units per KLK1 molecule. Also provided are mixtures of such glycoforms, pharmaceutical compositions containing such glycoforms or mixtures thereof, methods of obtaining the rhKLK1 glycoforms, and therapeutic uses thereof.

主权项

1. A composition comprising a first tissue kallikrein-1 (KLK1) polypeptide and a second tissue kallikrein-1 (KLK1) polypeptide:
wherein the first KLK1 polypeptide has three glycans attached at three different positions per said first KLK1 polypeptide and the second KLK1 polypeptide has two glycans attached at two different positions per said second KLK1 polypeptide; wherein the three glycans of the first KLK1 polypeptide are Asn-linked glycans at residues 78, 84, and 141 as defined by SEQ ID NO:1; wherein the two glycans of the second KLK1 polypeptide are Asn-linked glycans at residues 78 and 84 but not 141 as defined by SEQ ID NO:1; and wherein the first KLK1 polypeptide and the second KLK1 polypeptide are present in the composition in a ratio ranging from about 45:55 to about 55:45.