INTERCALATING DYES FOR DIFFERENTIAL DETECTION

摘要

Methods and compositions are provided for detection and quantification of nucleic acid sequences.

主权项

1. A nucleic acid sequence detection method comprising:
providing a sample comprising a DNA or RNA nucleic acid; partitioning said sample into a set of mixture partitions; detecting a presence or absence of a target nucleic acid in the partitions using a sequence specific detection reagent; and detecting a presence or absence of double-stranded nucleic acid in the partitions using a non-specific detection reagent,thereby detecting the ratio of target nucleic acid to total nucleic acid in the partitions.