PROTEIN BASED PRODUCT FROM FENUGREEK SEEDS THAT REGULATES DYSLIPIDEMIA AND OBESITY, AND A PROCESS FOR THE PREPARATION THEREOF

摘要

This invention relates to a protein based product from fenugreek seeds (TEFS), comprising: Grinding of dried fenugreek seeds suspending ground fine powder of the fenugreek seeds in PBS, pH 7.4 containing 0.1 mM PMSF and protease inhibitor cocktail under chilled conditions; filtering the extract through three layered cheese cloth, centrifuging; treating clear supernatant with activated charcoal powder; extracting or precipitating protein; removing the precipitate by centrifugation, re-dissolving in PBS and dialyzing against PBS; heat treating the dialyzed protein rich extract; removing the precipitated protein by centrifugation and retrieving supernatant as final product. Said final product has a protein concentration of 0.1-15 mg/ml, preferably 1-1.5 mg/ml. Said product is useful in the management of dyslipidemia and obesity.