发明名称 |
SOLID PHASE GLYCAN AND GLYCOPEPTIDE ANALYSIS AND MICROFLUIDIC CHIP FOR GLYCOMIC EXTRACTION, ANALYSIS AND METHODS FOR USING SAME |
摘要 |
Highly specific and novel solid phase methods for analyzing glycans and proteoglycans using a solid phase system are provided. The present invention also provides an integrated apparatus and methods of use which comprises a high-throughput glycan isolation and reverse-phase liquid chromatography (RPLC) for on-chip glycan extraction, modification and separation. The coverage of detected N-glycans by the GIG-chip-LC apparatus of the present invention can be significantly improved, especially for the low abundant species. Chip-LC by PGC minimizes dynamic range of glycan concentrations in fractions, resulting in detection of low-abundance glycans. Glycan isomers were able to be separated by the chip-LC portion of the apparatus. The GIG-chip-LC apparatus of the present invention can be used to analyze glycans from tissue and sera samples, thus providing a reliable tool for glycomic analysis. The reproducible performance and ability to detect unique glycans from tissue samples provides a powerful means for discovery of abnormal glycans associated with disease states. |
申请公布号 |
EP2892908(A1) |
申请公布日期 |
2015.07.15 |
申请号 |
EP20130835390 |
申请日期 |
2013.09.10 |
申请人 |
THE JOHNS HOPKINS UNIVERSITY |
发明人 |
ZHANG, HUI;YANG, SHUANG;SHAH, PUNIT;SUN, SHISHENG |
分类号 |
C07H1/06;C08B37/00;G01N33/53 |
主分类号 |
C07H1/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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