| 摘要 |
A method of quantitation of compounds of interest within a sample comprises performing a liquid chromatography separation of the sample, passing the liquid chromatography eluant into a mass spectrometer and performing ion mobility analysis and mass spectrometry analysis on the sample. Compounds of interest suspected to be present in the sample which coelute in the liquid chromatography separation and have substantially the same mass are identified. Data corresponding to each of the compounds of interest are identified by ion mobility separation of the compounds, and quantitation is performed on the compounds of interest from the data. The compounds of interest may be isomers, protomers, epimers or conformers. If the compounds of interest are only separated partially by ion mobility, the method may further comprise obtaining fragment mass spectrometry data by repeatedly switching modes of operation and using the intensities of fragment ions relating to the compounds of interest to provide quantitation information. |
