Establishment of motif comprising acidic amino acid, capable of stabilizing protein in cells, and applicable to protein therapy, control of differentiation/undifferentiation of cell and antibody therapy

摘要

A motif is searched which can inhibit the proteolysis of a protein that has been administered to a cell or an individual. Thus, disclosed is a method for designing/producing a protein having resistance to proteolysis. Specifically disclosed is a motif capable of inhibiting proteolysis, which comprises an amino acid region lying between the 396th position and the 410th position from the C-terminal of DP-1.

主权项

1. A fusion protein which has an acquired proteolysis resistance, the fusion protein comprising an inducer for inducing a somatic cell into an induced pluripotent stem cell (iPS cell), the inducer being Oct4, Sox2, or Klf4,
wherein a proteolysis-inhibiting motif is fused to at least one of the N-terminal and the C-terminal of the inducer, and wherein the proteolysis-inhibiting motif comprises the amino acid sequence of SEQ ID NO:1.