| 发明名称 |
Method of cryopreserving stem cells |
| 摘要 |
A non-linear cooling cryopreservation method for improving cryopreservation protocols for cells that involves producing a simulation of cellular responses to a range of cooling parameters; determining optimal cooling parameters required to minimize cryoinjury to the cells using simulation of cellular responses and experimental results; and incorporating optimal parameters into the protocol. The simulation is based on mathematical models of cellular parameters. A non-linear cooling cryopreservation protocol for cryopreserving stem cells is also disclosed that does not require cryoprotectants. |
| 申请公布号 |
US9078429(B2) |
申请公布日期 |
2015.07.14 |
| 申请号 |
US201012656600 |
申请日期 |
2010.02.04 |
| 申请人 |
The Governors of the University of Alberta |
发明人 |
McGann Locksley Earl;Elliott Janet Anne Wade;Ross-Rodriguez Lisa Ula |
| 分类号 |
C12N5/00;A01N1/02 |
主分类号 |
C12N5/00 |
| 代理机构 |
|
代理人 |
Lambert Anthony R. |
| 主权项 |
1. A method for cryopreserving stem cells, the method comprising:
providing isolated stem cells in a serum-free liquid culture medium; a. Cooling the stem cells to a first temperature between −5° C. and −15° C. and holding them for a first period of time between 1 and 30 minutes at said first temperature to generate ice outside of the stem cells; and thereafter b. Cooling the stem cells to a second temperature below −60° C. for storing the cells; wherein steps a and b are carried out in the absence of a permeating cryopreservant. |
| 地址 |
Edmonton, Alberta CA |
