Using populations of beads for the fabrication of arrays on surfaces

摘要

The present invention provides methods for creating an array of features on a surface based on content transferred from a plurality of beads to the surface. Nucleic acid content can be transferred using a method including the steps of (a) providing a surface having one or more primer oligonucleotides attached to the surface; (b) providing a pool of beads, wherein beads in the pool have a plurality of templates attached thereto, the plurality comprising multiple copies of a single nucleic acid template sequence; (c) arraying the beads onto the surface by hybridizing the templates to the primer oligonucleotides; and (d) extending the primers to produce copies of the templates attached to the surface.

主权项

1. An array comprising spatially discrete nucleic acid features on a surface, wherein each spatially discrete feature comprises two or more different amplification primers each having a different sequence attached to the surface, and wherein each spatially discrete feature is separated from any other discrete feature by a space which does not carry amplification primers, wherein the array of spatially discrete features comprises an average center-to-center distance that is less than two times the diameter of a feature.