Method of measuring glycosylated protein proportion by AC impedance method

摘要

A method of measuring a glycosylated protein proportion by an AC impedance method includes passing an AC voltage or AC to two inspecting electrodes; covering a solution containing glycosylated protein completely onto the inspecting electrodes; measuring an impedance value between the electrodes and calculating the difference between the impedance value and an impedance value of a solution without glycosylated protein by an AC impedance inspection device; comparing the impedance difference with AC impedance difference/glycosylated protein concentration to obtain the proportion of the glycosylated protein in the solution containing glycosylated protein. The solution without glycosylated protein includes a salt solution and a non-glycosylated protein; the proportion of glycosylated protein in the solution containing glycosylated protein refers to the proportion of glycosylated protein in the total quantity of protein in the salt solution; and the proportion of glycosylated hemoglobin of the total quantity of hemoglobin in the salt solution.

主权项

1. A method of measuring a glycosylated protein proportion by and AC impedance method, comprising the steps of:
passing an alternating current (AC) voltage or an alternating current (AC) to a pair of inspecting electrodes, and covering the inspecting electrodes completely by a solution containing a glycosylated protein, and obtaining an impedance value between the electrodes through an AC impedance inspection device; calculating the impedance difference between the impedance value and an impedance value of a salt and non-glycosylated protein solution without glycosylated protein; creating a plurality of reference curves defining the relationship between the concentration of glycosylated protein and the impedance difference for a range of AC frequencies; and comparing the impedance difference with a relation of AC impedance difference to glycosylated protein concentration to obtain the proportion of the glycosylated protein in the solution containing the glycosylated protein.