发明名称 |
Production cell line enhancers |
摘要 |
The present invention relates to discovery of the ectopic expression of EDEM2 in a production cell to improve the yield of a useful multi-subunit protein. Thus, the present invention provides for production cell lines, such as the canonical mammalian biopharmaceutical production cell—the CHO cell, containing recombinant polynucleotides encoding EDEM2. Also disclosed is a production cell containing both an EDEM2-encoding polynucleotide as well an XBP1-encoding polynucleotide. Improved titers of antibodies produced by these cell lines are disclosed, as well as the improved cell densities attained by these cells in culture. |
申请公布号 |
US9079954(B2) |
申请公布日期 |
2015.07.14 |
申请号 |
US201313904587 |
申请日期 |
2013.05.29 |
申请人 |
REGENERON PHARMACEUTICALS, INC. |
发明人 |
Chen Gang;Burakov Darya;Deshpande Dipali |
分类号 |
C12N5/07;C12N5/16;C07H21/02;C07H21/04;C07K16/22;C12N15/79;C12N15/85 |
主分类号 |
C12N5/07 |
代理机构 |
|
代理人 |
Johnson Mary C.;Zahner Joseph |
主权项 |
1. An ex vivo mammalian cell comprising a polynucleotide, which comprises a nucleic acid sequence of SEQ ID NO: 16. |
地址 |
Tarrytown NY US |