| 摘要 |
<p>The invention relates to a simple, accurate, specific, sensitive and precise RP-HPLC method for the estimation of P-sitosterol in the Cynodon dactylon. The method of the present invention is validated as per the ICH guideline. The method comprising the steps of preparing the standard solution of P-sitosterol and the sample solution of Cynodon dactylon extract, running the standard solution and sample solution into the HPLC system. RP-HPLC was carried out using acetonitrile: methanol (80:20) v/v as the mobile phase at 202 nm wavelength and 1 ml/min flow rate using C-18 column of particle size 5 um, inner diameter 4.6 mm and 250 mm length. The present invention also relates to novel method for standardization of Cynodon dactylon.</p> |
