BIOSENSOR FOR DETECTION OF MYCOTHIOL REDOX POTENTIAL.

摘要

The disclosure provides a fusion construct that includes an engineered green fluorescent protein having a fluorescence spectrum that is sensitive to redox status and a mycothiol-dependent oxidoreductase linked to the engineered green fluorescent protein. The mycothiol-dependent oxidoreductase is a mycoredoxin identified from Mycobacterium tuberculosis strain H37Rv and the fusion construct can be used as a biosensor for detecting a change in a redox status of mycothiol in a cell.