| 摘要 |
<p>ABSTRACT ISOLATION, IDENTIFICATION, CHARACTERIZATION AND BIOEFFICACY TESTING OF 3, 4-SECO-LUP-20(29)-EN-3-OIC ACID, NOVEL DIABETIC THERAPEUTIC AGENTS FROM SEED OF HOLARRHENA ANTIDYSENTERICA A method of obtaining anti-diabetic/anti-hyperglycemic novel compound 3, 4-seco-lup-20(29)-en-3-oic acid of formula I, I I said method comprising maceration of a certain weight of pulverized seeds of Holarrhena antidysenterica at room temperature suitably in a solvent and preparation of lyophilized extract, dissolving the lyophilized crude extract in a suitable volume of hydroethanolic solution, carrying out solvent partitioning using various solvents, subjecting all fractions obtained by filtration for the bio-activity study to identify most active fraction responsible for anti-hyperglycemic/anti-diabetic activity and subjecting it to column chromatography, the sticky solid mass so obtained is crystallized twice from 20% chloroform in n-Hexane solvent to yield colourless rectangular crystals, identified as compound with structural formula I.</p> |
