COMPOSITION AND METHOD FOR SEQUENCING NUCLEIC ACID

摘要

A composition for sequencing DNA is provided and comprises a nuclease and a nuclease-resistant sequencing primer. A method of preparing DNA for sequencing and a method of sequencing DNA are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition under conditions in which excess amplification primer is degraded by the nuclease and the nuclease-resistant sequencing primer is essentially non-degraded.

主权项

1. A method comprising the steps of:
amplifying a DNA sequence under conditions to produce an amplification reaction product, the amplification reaction product comprising an excess amplification primer; and contacting the amplification reaction product with a reaction mixture comprising an exonuclease I and a sequencing primer configured to be resistant to degradation by the exonuclease I, whereby, the excess amplification primer is degraded by the exonuclease I and the nuclease-resistant sequencing primer is essentially non-degraded.