发明名称 |
SELF-IMMOLATIVE PROBES FOR ENZYME ACTIVITY DETECTION |
摘要 |
Provided is a compound comprising the structure:;(SIG)-(SI-MOD)m.;In this compound, SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. With this compound, when MOD is modified by an activator, SI is destabilized and self cleaved from SIG such that SIG generates an increased signal. Also provided is a method of determining whether a sample comprises an activator, using the above-described compound. Additionally provided is a method of determining whether a cell comprises a nitroreductase using the above-described compound where nitroreductase is the activator. Further provided is a method of determining whether a mammalian cell is hypoxic using the above-described compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase, using the above-described compound where nitroreductase is the activator, is also provided. Also provided is a method of identifying nitroreductase in a sample, using the above-described compound where nitroreductase is the activator. |
申请公布号 |
US2015191768(A1) |
申请公布日期 |
2015.07.09 |
申请号 |
US201414447817 |
申请日期 |
2014.07.31 |
申请人 |
Enzo Life Sciences, Inc. c/o Enzo Biochem, Inc. |
发明人 |
SZCZEPANIK MACIEJ;LEBEDEVA IRINA V.;XIANG YUEJUN;PANDE PRAVEEN;PATTON WAYNE FORREST |
分类号 |
C12Q1/48;C12Q1/44;C12Q1/37 |
主分类号 |
C12Q1/48 |
代理机构 |
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代理人 |
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主权项 |
1. A compound comprising the structure:
(SIG)-(SI-MOD)m wherein SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10, wherein, when MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. |
地址 |
NEW YORK NY US |