PEGYLATED TISSUE KALLIKREIN, AND PREPARATION METHOD THEREFOR AND USES THEREOF

摘要

The present invention relates to polyethylene glycol modified protein medicaments. Disclosed are pegylated tissue kallikrein, a preparation method therefor, and uses thereof. The tissue kallikrein has a sequence shown in SEQ ID No. 1 or SEQ ID No. 2, the tissue kallikrein can be natural or recombinant, polyethylene glycol is polyethylene glycol with a molecular weight of 20-40kDa, and is coupled on an N-terminal primary amine of the tissue kallikrein. The pegylated KLK1 provided in the present invention has the advantages of significantly-extended life period, significantly-reduced immunogenicity and a stable and uniform structure, can be used for improving activities of the pegylated tissue kallikrein to a higher extent, and particularly, has more significant biological activities in the aspect of treating cerebral apoplexy and diabetic nephropathy.