| 发明名称 |
BIOLUMINESCENT ASSAYS UTILISING SECRETED LUCIFERASES |
| 摘要 |
Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase. |
| 申请公布号 |
US2015191769(A1) |
申请公布日期 |
2015.07.09 |
| 申请号 |
US201514660413 |
申请日期 |
2015.03.17 |
| 申请人 |
Leu Marco Peter;Daly John Michael |
发明人 |
Leu Marco Peter;Daly John Michael |
| 分类号 |
C12Q1/66;C12N9/99 |
主分类号 |
C12Q1/66 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for measuring the luminescent signal generated by a luciferase in a sample, wherein the luciferase, in its native form, is a secreted luciferase, the method comprising:
(a) contacting the sample with a reactive substrate of the luciferase; (b) contacting the sample with a reducing agent that time dependently inactivates the luciferase; and (c) measuring the luminescent signal generated by the luciferase before the luciferase is inactivated by the reducing agent. |
| 地址 |
East Perth AU |
