Compositions and Methods for Modulating Biomass Productivity

摘要

The disclosure generally relates to methods and materials for modulating cell productivity. In particular, the present disclosure provides polynucleotides encoding transcription factor proteins that when overexpressed in microrganisms result in increased in productivity, such as increased biomass productivity. Also disclosed are methods of using the genetically engineered host strains to modulate or increase productivity of host cells such as, for example, algal or heterokont cells. Genetically engineered host cells, such as algal and heterokont cells having increased biomass productivity and bioproducts derived from such host cells are also disclosed.

主权项

1. A recombinant host cell comprising a non-native nucleic acid molecule encoding a HAP3-like polypeptide that includes an amino acid sequence having at least 65% identity to an amino acid sequence selected from the group consisting of: SEQ ID NO:4; amino acids 27-117 of SEQ ID NO:8; amino acids 23-113 of SEQ ID NO:10; amino acids 24-114 of SEQ ID NO:12; amino acids 24-114 of SEQ ID NO:14; amino acids 54-144 of SEQ ID NO:16, amino acids 19-109 of SEQ ID NO:18, amino acids 15-105 of SEQ ID NO:20, amino acids 18-108 of SEQ ID NO:22, amino acids 26-116 of SEQ ID NO:24, amino acids 29-119 of SEQ ID NO:26, amino acids 26-116 of SEQ ID NO:28, amino acids 23-113 of SEQ ID NO:30, amino acids 26-116 of SEQ ID NO:32, amino acids 20-110 of SEQ ID NO:34, amino acids 16-106 of SEQ ID NO:36, amino acids 24-114 of SEQ ID NO:38, amino acids 21-111 of SEQ ID NO:40, amino acids 29-119 of SEQ ID NO:42, amino acids 20-110 of SEQ ID NO:44, amino acids 20-110 of SEQ ID NO:46, amino acids 9-96 of SEQ ID NO:50, and amino acids 16-106 of SEQ ID NO:52;
wherein the recombinant host cell has increased productivity with respect to a control cell that does not include the non-native nucleic acid molecule encoding a HAP3-like polypeptide.