发明名称 PCV2 IMMUNOGENIC COMPOSITIONS AND METHODS OF PRODUCING SUCH COMPOSITIONS
摘要 An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.
申请公布号 US2015190497(A1) 申请公布日期 2015.07.09
申请号 US201514661689 申请日期 2015.03.18
申请人 BOEHRINGER INGELHEIM VETMEDICA, INC. 发明人 Eichmeyer Marc;Nitzel Greg;Schaeffer Merrill
分类号 A61K39/12;G01N33/569;C07K14/005;C12N7/00 主分类号 A61K39/12
代理机构 代理人
主权项 1. A method of recovering recombinant protein expressed by open reading frame 2 of PCV2 comprising the steps of: A) cloning said recombinant open reading frame 2 from PCV2 into a transfer vector; B) transfecting the portion of said transfer vector containing said recombinant open reading frame 2 into a virus; C) infecting cells in media with said virus; D) causing said virus to express the protein from said open reading frame 2; E) separating cells from said vector from the supernate; and F) recovering said expressed open reading frame 2 protein in said supernate.
地址 ST. JOSEPH MO US