共有結合形成反応ペアを用いた標的分子の粒子ベースの検出における方法および組成物

摘要

Methods and compositions which can be used to increase the strength and/or probability of forming a binding complex comprising a target molecule and a substrate are disclosed. In one aspect, linking molecules having one or more members of a reactive pair are disclosed which can be used to increase the number of intra-complex binding interactions in a complex comprising a target molecule and a substrate. Intra-complex crosslinking and inter-complex crosslinking can be utilized in connection with these methods and compositions to further strengthen and stabilize the disclosed binding complexes.