| 摘要 |
A nucleic acid analysis device which can determine a DNA sequence at a long read base length by highly parallel processing with high accuracy is provided. The nucleic acid analysis device of the present invention has a flowcell in which two or more DNA fragment clusters of two or more DNA fragments having identical nucleotide sequences are immobilized, wherein at least a part of the flowcell is made of a transparent material, an irradiation unit for irradiating a part in which the DNA fragment clusters are immobilized, a lens for collecting fluorescence, and a light-detection element for detecting a collected light: which is characterized in that a solution containing only dATP having a fluorescently modified phosphate terminal among four bases, a solution containing only dCTP having a fluorescently modified phosphate terminal among the four bases, a solution containing only dGTP having a fluorescently modified phosphate terminal among the four bases, a solution containing only dTTP having a fluorescently modified phosphate terminal among the four bases, and a buffer solution for washing out the bases are sent sequentially to the part in which the DNA fragment clusters are immobilized. |
