| 摘要 |
<p>PROBLEM TO BE SOLVED: To provide a screening method and a proper evaluation system for developing a drug uniformly having cell-death inducing activity on all cancer cells, or a drug preventing the all cancer cells from proliferating, or for developing an assistant drug for making a known anticancer agent evenly act on the all cells, or an assistant drug for recovering sensitivity of known anticancer agent resistant strains.SOLUTION: Cancer cell population is seminated at a low density and cultured to form a colony on each cancer cell. Then a test drug is added, and a cell image is captured with time and compared with a result attained without adding the test drug to calculate the proliferation rate of the cell and cell-death inducing rate of the test drug accurately on each colony. The average values of the proliferation rate of the cell and the cell-death inducing rate of the whole cell population are calculated from the values of each colony, together with variation coefficient (C.V.) of both data for each cell, and these values are calibrated by a control to evaluate the efficacy as an anticancer agent or as an assistant drug of known anticancer agents. Meanwhile adverse effect degree on normal cells is evaluated by performing contrast experiment when using normal cells in the case of screening the anticancer agents in conjunction with contrast experiment when added with only the assistant drug in the case of screening the assistant drug, and then by calculating the average value of the proliferation rate of the cell and the cell-death inducing rate of the whole cell population, together with variation coefficient (C.V.).</p> |
