发明名称 |
Burdock fruit extract containing arctigenin and method for producing same |
摘要 |
PROBLEM TO BE SOLVED;The present invention is intended to provide a burdock fruit extract comprising arctigenin and arctiin at a definite ratio and a method for producing the same. More particularly, the present invention is intended to provide a method for producing a burdock fruit extract comprising arctigenin and arctiin at a weight ratio of approximately 1:1.;SOLUTION;A method for producing a burdock fruit extract comprising arctigenin and arctiin at a weight ratio of arctigenin/arctiin=0.7 to 1.3 is provided, including the steps of: cutting a burdock fruit and converting arctiin which is inherent in the burdock fruit into arctigenin by enzymatic conversion by beta-glucosidase which is inherent in the burdock fruit, wherein the enzymatic conversion includes reaction at a temperature from 20° C. to 50° C. Also provided is the method further including a step of extracting an extract comprising arctigenin and arctiin by adding an organic solvent and heating to reflux. |
申请公布号 |
US9072718(B2) |
申请公布日期 |
2015.07.07 |
申请号 |
US201313849302 |
申请日期 |
2013.03.22 |
申请人 |
National University Corporation University of Toyama;Kracie Pharma, Ltd.;National Cancer Center |
发明人 |
Okubo Toshiki;Yomoda Satoshi;Fuse Takafumi;Kawashima Takanori;Esumi Hiroyasu;Miyoshi Chika;Kadota Shigetoshi |
分类号 |
A01N65/00;A61K36/28;A61K31/365;A61K31/7048;C07D307/33 |
主分类号 |
A01N65/00 |
代理机构 |
Sunstein Kann Murphy & Timbers LLP |
代理人 |
Sunstein Kann Murphy & Timbers LLP |
主权项 |
1. A method for producing a burdock fruit extract containing arctigenin and arctiin at a weight ratio of arctigenin/arctiin=0.7 to 1.3 from a burdock fruit which inherently comprises arctiin and beta-glucosidase, the method comprising the steps of:
cutting a burdock fruit, adding water to said cut burdock fruit, and stirring said cut burdock fruit to a temperature of 20° C. to 50° C. to permit enzymatic conversion of arctiin into arctigenin by beta-glucosidase, to produce a burdock fruit extract, wherein the enzyme activity of said beta-glucosidase is 0.4 U or greater per 1 g of said burdock fruit, and further comprising an extraction step which comprises adding an organic solvent to said burdock fruit extract, wherein the burdock fruit is retained at a temperature of 80° C. or higher during said extraction step to yield the burdock fruit extract containing arctigenin and arctiin at a weight ratio of arctigenin/arctiin=0.7 to 1.3 from a burdock fruit which inherently comprises arctiin and beta-glucosidase. |
地址 |
Toyama JP |