发明名称 Burdock fruit extract containing arctigenin and method for producing same
摘要 PROBLEM TO BE SOLVED;The present invention is intended to provide a burdock fruit extract comprising arctigenin and arctiin at a definite ratio and a method for producing the same. More particularly, the present invention is intended to provide a method for producing a burdock fruit extract comprising arctigenin and arctiin at a weight ratio of approximately 1:1.;SOLUTION;A method for producing a burdock fruit extract comprising arctigenin and arctiin at a weight ratio of arctigenin/arctiin=0.7 to 1.3 is provided, including the steps of: cutting a burdock fruit and converting arctiin which is inherent in the burdock fruit into arctigenin by enzymatic conversion by beta-glucosidase which is inherent in the burdock fruit, wherein the enzymatic conversion includes reaction at a temperature from 20° C. to 50° C. Also provided is the method further including a step of extracting an extract comprising arctigenin and arctiin by adding an organic solvent and heating to reflux.
申请公布号 US9072718(B2) 申请公布日期 2015.07.07
申请号 US201313849302 申请日期 2013.03.22
申请人 National University Corporation University of Toyama;Kracie Pharma, Ltd.;National Cancer Center 发明人 Okubo Toshiki;Yomoda Satoshi;Fuse Takafumi;Kawashima Takanori;Esumi Hiroyasu;Miyoshi Chika;Kadota Shigetoshi
分类号 A01N65/00;A61K36/28;A61K31/365;A61K31/7048;C07D307/33 主分类号 A01N65/00
代理机构 Sunstein Kann Murphy & Timbers LLP 代理人 Sunstein Kann Murphy & Timbers LLP
主权项 1. A method for producing a burdock fruit extract containing arctigenin and arctiin at a weight ratio of arctigenin/arctiin=0.7 to 1.3 from a burdock fruit which inherently comprises arctiin and beta-glucosidase, the method comprising the steps of: cutting a burdock fruit, adding water to said cut burdock fruit, and stirring said cut burdock fruit to a temperature of 20° C. to 50° C. to permit enzymatic conversion of arctiin into arctigenin by beta-glucosidase, to produce a burdock fruit extract, wherein the enzyme activity of said beta-glucosidase is 0.4 U or greater per 1 g of said burdock fruit, and further comprising an extraction step which comprises adding an organic solvent to said burdock fruit extract, wherein the burdock fruit is retained at a temperature of 80° C. or higher during said extraction step to yield the burdock fruit extract containing arctigenin and arctiin at a weight ratio of arctigenin/arctiin=0.7 to 1.3 from a burdock fruit which inherently comprises arctiin and beta-glucosidase.
地址 Toyama JP