Mutant hydroxyphenylpyruvate dioxygenase polypeptides and methods of use

摘要

Compositions and methods for conferring hydroxyphenyl pyruvate dioxygenase (HPPD) herbicide resistance or tolerance to plants are provided. Compositions include amino acid sequences, and variants and fragments thereof, for mutant HPPD polypeptides. Nucleic acids that encode the mutant HPPD polypeptides are also provided. Methods for conferring herbicide resistance or tolerance, particularly resistance or tolerance to certain classes of herbicides that inhibit HPPD, in plants are further provided. Methods are also provided for selectively controlling weeds in a field at a crop locus and for the assay, characterization, identification and selection of the mutant HPPDs of the current invention that provide herbicide tolerance.

主权项

1. A polynucleotide encoding a polypeptide having at least 85% sequence identity to SEQ ID NO:27, wherein said polypeptide is plant-derived and has 4-hydroxyphenylpyruvate dioxyqenase (HPPD) enzymatic activity and comprises the amino acid sequence RFDHVVGNV (SEQ ID NO:38), wherein the first V of SEQ ID NO: 38 is replaced with any other amino acid.