| 发明名称 | Recombinant factor VIII having increased stability | ||
| 摘要 | The present invention relates to a recombinant factor VIII that includes one or more mutations that result in enhanced stability of both factor VIII and factor VIIIa. Methods of making and using the recombinant factor VIII, and pharmaceutical compositions containing the same are also disclosed. The present invention further relates to an isolated nucleic acid molecule that encodes the recombinant factor VIII, as well as DNA expression systems and host cells containing the isolated nucleic acid molecule. | ||
| 申请公布号 | US9072722(B2) | 申请公布日期 | 2015.07.07 |
| 申请号 | US201213691474 | 申请日期 | 2012.11.30 |
| 申请人 | University of Rochester | 发明人 | Fay Philip J.;Wakabayashi Hironao |
| 分类号 | A61K38/37;C07K14/755 | 主分类号 | A61K38/37 |
| 代理机构 | LeClairRyan, a Professional Corporation | 代理人 | LeClairRyan, a Professional Corporation |
| 主权项 | 1. A recombinant human factor VIII comprising a substitution or substitutions of one or more charged amino acid residues with a hydrophobic amino acid residue at either or both of the A1 A2 or A2A3 domain interfaces, wherein the one or more charged amino acid residues is not inter-domain hydrogen bonded in a wildtype human factor VIII, and wherein the substitution or substitutions result in enhanced stability of both recombinant factor VIII and recombinant factor VIIIa. | ||
| 地址 | Rochester NY US | ||