Tuberculosis TB vaccine to prevent reactivation

摘要

The present invention discloses a vaccine or immunogenic composition that can be administered to latently infected individuals to prevent reactivation of latent tuberculosis infection caused by species of the tuberculosis complex microorganisms (Mycobacterium tuberculosis, M. bovis, M. africanum). The invention is base on a number of M. tuberculosis derived proteins and protein fragments which are constitutively expressed in different stages of the infection. The invention is directed to the use of these polypeptides, immunologically active fragments thereof and the genes encoding them for immunological compositions such as vaccines.

主权项

1. A vaccine comprising an M. tuberculosis antigen polypeptide, which is constitutively expressed during infection with M. tuberculosis or a nucleic acid encoding said M. tuberculosis antigen polypeptide, wherein said vaccine prevents reactivation of tuberculosis in latently infected individuals, wherein the vaccine further comprises an adjuvant comprising IC31 or DDA/TDB, with or without poly I:C, and wherein the M. tuberculosis antigen polypeptide, which is constitutively expressed, belongs to the ESX-1 secretion system and comprises:
i) a first polypeptide sequence set forth in any one of SEQ ID NO. 3, 4, 5, 6, 7, 8, 9, 14, 15, 16, 17, 18, 32, 33, or 34; or ii) a second polypeptide sequence that comprises at least 80% sequence identity to any one of the sequences in (i), wherein said second polypeptide sequence is immunogenic.