Culture medium additive for use in serum-free culturing of animal cell, kit and use thereof

摘要

Disclosed are: a culture medium containing a specific growth factor and at least one phospholipid; a composition for preparation of the culture medium; a kit; and a method. A technique can be provided which uses a serum-free or low-serum culture medium and has a promoting effect on the proliferation of an animal cell comparable to the promoting effect obtained by the culture in a serum-containing culture medium.

主权项

1. A culture medium additive for use in serum-free culturing of a mesenchymal stem cell or a monkey kidney-derived undifferentiated cell line COS7 cell, the culture medium additive consisting of:
(a) FGF, PDGF, TGF-β, and HGF; (b) two or more factors selected from the group consisting of EGF, CTGF, VEGF and ascorbic acid compound; (c) one or more phospholipids; (d) one or more fatty acids; (e) one of the following components (i) to (iv):
(i) PLURONIC F-68 (polyoxyethylene-polyoxypropylene block copolymer) or TWEEN-80 (polyoxyethylene sorbitan monooleate),(ii) dexamethasone,(iii) PLURONIC F-68 (polyoxyethylene-polyoxypropylene block copolymer) and dexamethasone, or TWEEN-80 (polyoxyethylene sorbitan monooleate) and dexamethasone, and(iv) PLURONIC F-68 (polyoxyethylene-polyoxypropylene block copolymer) and dexamethasone, or TWEEN-80 (polyoxyethylene sorbitan monooleate); (f) optionally, one or more of the following components (v) to (viii):
(v) cholesterol,(vi) a lipid oxidation inhibitor,(vii) lithium chloride, and(viii) insulin, transferrin and selenate.