Method for evaluating the improvement of skin elasticity using a mimetic dermis

摘要

The present invention relates to a method for evaluating the improvement of skin elasticity by preparing a mimetic dermis having a structure similar to that of the real dermis, and providing a three-dimensional visualization of the differences in the degree of transformation of the mimetic dermis.

主权项

1. A method for quantifying improvement in skin elasticity, the method comprising the steps of:
a) preparing (i) a glycated collagen control group by reaction of collagen with sugar without a candidate whose skin elasticity-improving effect is to be evaluated and (ii) a glycated collagen test group by reaction of collagen and sugar with the candidate, and mixing fibroblasts with each of the control group and the test group to make aqueous collagen solution; b) constructing three-dimensional shapes by placing each aqueous collagen solution of the control group and the test group in culture inserts to harden as gels; c) culturing each of the gels of the control group and the test group in their culture inserts of step b) to prepare dermal mimics; d) obtaining three-dimensional images with a three-dimensional imaging device of the three dimensional shapes of the prepared mimics after separation from their culture inserts; and e) measuring a change of at least thickness or volume of the prepared mimics from the images of step d).