Method for production of chrysanthemum plant having delphinidin-containing petals

摘要

Disclosed are: a method for producing a chrysanthemum plant having delphinidin-containing petals using a transcriptional regulatory region for a chrysanthemum-derived flavanone 3-hydroxylase (F3H) gene; and a chrysanthemum plant, a progeny or a vegetative proliferation product of the plant, or a part or a tissue of the plant, the progeny or the vegetative proliferation product, and particularly a petal or a cut flower of the plant. In the method for producing a chrysanthemum plant having delphinidin-containing petals, a flavonoid 3′,5′-hydroxylase (F3′5′H) is caused to be expressed in a chrysanthemum plant using a transcriptional regulatory region for a chrysanthemum-derived flavanone 3-hydroxylase (F3H) gene.

主权项

1. A method for producing a chrysanthemum plant containing delphinidin in the petals thereof comprising expressing flavonoid 3′,5′-hydroxylase (F3′5′H) using a transcriptional regulatory region; wherein the chrysanthemum plant is transformed with an expression vector or expression cassette comprising a gene encoding F3′5′H and the transcriptional regulatory region; wherein the F3′5′H is derived from bellflower (campanula), cineraria, verbena, or pansy; and wherein the transcriptional regulatory region is
a nucleic acid containing the nucleotide sequence indicated in SEQ ID NO: 34 or SEQ ID NO: 87.