ADSORBENT, METHOD FOR PRODUCING ADSORBENT, AND ANTIBODY PURIFICATION METHOD

摘要

PROBLEM TO BE SOLVED: To provide an adsorbent in which the shrinkage of the adsorbent is suppressed, maintaining DBC (Dynamic Binding Capacity) of antibodies in affinity chromatography using temperature-responsive protein A.SOLUTION: The invention provides an adsorbent in which a polypeptide containing a B domain variant of protein A is bound to a carrier, wherein the volume (A) when contacting to 20 mmol/L of a phosphate buffer solution (pH 8.0) and a volume (B) when contacting to 50 mmol/L of a citrate buffer solution (pH 4.0) fill the following formula (1). ((A-B)/A)<0.025 ... (1)