| 摘要 |
<p>The present invention relates to a method for analyzing an autosome of a human subject based on a next generation sequencing (NGS) technology, wherein the method comprises the steps of: (a) performing multiplex amplification by using primers that complementarily bind, respectively, to D3S1358, TH01, D21S11, D18S51, PentaE, D5S818, D13S317, D7S820, D16S539, CSF1PO (Human c-fms proto-oncogene for CSF-1 receptor gene), PentaD, von Willebrand factor A (vWA), D8S1179, Human thyroid peroxidase gene (TPOX), Human fibrinogen alpha chain (FGA), and amelogenin loci of a human subject DNA sample; (b) determining the short tandem repeat (STR) allele of each of the loci by using NGS data of the product of the multiplex amplification in the step (a), and identifying the human subject with a gene. The method using an NGS system according to the present invention can be used for analyzing and interpreting an STR profile from a single-source sample, mixed sample, and decomposed DNA sample in forensic science.</p> |
