| 摘要 |
Eukaryotic cells, preferably a yeast or a filamentous fungus, with the ability to convert L-arabinose into D-xylulose 5-phosphate are provided, said ability acquired by transformation with nucleotide sequences coding for an arabinose isomerase, a ribulokinase, and a ribulose-5-P-4-epimerase from a bacterium of genus Clavibacter, Arthrobacter or Gramella. Preferably the can produce a fermentation product such as ethanol, lactic acid, 3-hydroxy-propionic acid, acrylic acid, acetic acid, succinic acid, citric acid, amino acids, 1,3-propane-diol, ethylene, glycerol, -lactam antibiotics and cephalosporins. The invention further relates to processes for producing these fermentation products wherein a cell of the invention is used to ferment arabinose into the fermentation products. |
| 主权项 |
1. A eukaryotic cell comprising a first, a second, and a third nucleotide sequence, the expression of which confers on the cell, or increases in the cell, the ability to convert L-arabinose to D-xylulose 5-phosphate, wherein:
(a) the first nucleotide sequence encodes an arabinose isomerase protein that comprises an amino acid sequence that is at least 90% identical to SEQ ID NO: 2; (b) the second nucleotide sequence encodes a ribulokinase protein that comprises an amino acid sequence that is at least 90% identical to SEQ ID NO: 6; and (c) the third nucleotide sequence encodes a ribulose-5-P-4-epimerase protein that comprises an amino acid sequence that is at least 90% identical to SEQ ID NO: 7. |
