发明名称 |
SCAVENGER CONTAINING PROTEIN FORMED FROM TANDEM-TYPE MULTIMER OF MUTANT EXTRACELLULAR DOMAIN OF PROTEIN G |
摘要 |
The purpose of the present invention is to provide a superior protein with more decreased binding property to the Fc region of immunoglobulin and/or more decreased binding property to the Fab region thereof in the weakly acidic region in comparison with a protein containing an extracellular domain of a wild-type protein G without spoiling the high antibody binding property in the neutral region, and to allow the antibody to be easily captured and recovered without denaturing it by means of a capturing agent and column comprising the above protein.;The present invention relates to a capturing agent for an antibody, immunoglobulin G or a protein comprising an Fc region of the immunoglobulin G, wherein a protein consisting of a tandem-type multimer of extracellular domain mutants that have binding property to the protein comprising the Fc region of the immunoglobulin G is immobilized to a water-insoluble solid support via mainly an amino group, and a column for a chromatography for separating and purifying the protein, which is filled with the capturing agent. |
申请公布号 |
US2015183820(A1) |
申请公布日期 |
2015.07.02 |
申请号 |
US201314416579 |
申请日期 |
2013.07.29 |
申请人 |
NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE TECHNOLOGY ;DAICEL CORPORATION |
发明人 |
Honda Shinya;Watanabe Hideki;Yoshida Chuya;Ueda Momoko;Nakai Yasuto;Isobe Yutaka |
分类号 |
C07K1/22;C07K17/08;C07K14/315 |
主分类号 |
C07K1/22 |
代理机构 |
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代理人 |
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主权项 |
1. A capturing agent for an antibody, immunoglobulin G or a protein comprising an Fc region of the immunoglobulin G, wherein a protein consisting of a tandem-type multimer of extracellular domain mutants that have binding property to the protein comprising the Fc region of the immunoglobulin G is immobilized to a water-insoluble solid support via mainly an amino group. |
地址 |
Tokyo JP |