发明名称 |
METHOD FOR PURIFYING ANTIBODY HAVING LOW ISOELECTRIC POINT |
摘要 |
The inventors of the present invention found that by using a protein A column to purify a low pI antibody, performing an acid treatment and a neutralisation treatment, and thereafter, when a fixed time period has elapsed, performing a step for eliminating antibody aggregates that have been produced, subsequent antibody aggregation can be suppressed. Furthermore, the inventors found that by purifying a low pI antibody in the bind/elute mode of an anion exchange resin and further using a hydrophobic interaction chromatography or multimodal chromatography resin, it is possible to eliminate impurities more efficiently than conventional methods. |
申请公布号 |
WO2015099165(A1) |
申请公布日期 |
2015.07.02 |
申请号 |
WO2014JP84671 |
申请日期 |
2014.12.26 |
申请人 |
CHUGAI SEIYAKU KABUSHIKI KAISHA |
发明人 |
UEDA, YASUFUMI;KOBAYASHI, SHOHEI;YANAGITA, SATOKO;KAWASE, TAKUO;FUKUNAGA, MASAHIRO |
分类号 |
C07K1/22;A61K39/395;C07K1/18;C07K1/20;C07K16/00 |
主分类号 |
C07K1/22 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|