COMPOSITIONS, METHODS AND KITS FOR NUCLEIC ACID SYNTHESIS AND AMPLIFICATION

摘要

The present invention is directed to compositions, methods and kits useful for the synthesis of nucleic acid molecules. More specifically, compositions, methods and kits are provided for the amplification of nucleic acid molecules in a one-step RT-PCR procedure comprising one or more agents used to increase tolerance to PCR inhibitors.

主权项

1. A composition comprising at least one DNA polymerase, at least one reverse transcriptase (RT), and at least one PCR inhibitor blocking agent, wherein said PCR inhibitor blocking agent increases tolerance to one or more PCR inhibitors.