METHODS AND SYSTEMS FOR REAL-TIME SENSING OF VIABLE AND NONVIABLE CELLS

摘要

Provided herein are techniques for identification of viable and nonviable cells in a cell culture that include measuring a resonance impedance spectral response of at least one resonator in proximity to the cell culture and correlating the measured response to the concentration of viable cells in cell culture and/or the concentration of nonviable cells in cell culture.

主权项

1. A method for analyzing a cell culture reaction fluid, comprising the steps of:
generating a plurality of frequencies with a sensor comprising a sensing region in operational contact with the cell culture reaction fluid and a plurality of tuning circuits outside the fluid; receiving a signal from the sensor, wherein the signal is representative of impedance spectra of the sensing region in operational contact with the cell culture reaction fluid over a measured spectral frequency range; analyzing the impedance spectra; and determining one or more properties of the cell culture reaction fluid based on the analyzed impedance spectra.