| 摘要 |
In order to determine the location (XM) of individual molecules of a substance in a sample, wherein the individual molecules of the substance are in a fluorescent state in which they are excitable with excitation light for emission of fluorescent light, and wherein distances between the individual molecules of the substance in a region of interest in the sample comply with a minimum value d = λ/(2nsinα √(1 + l/ls)), the individual molecules of the substance are excited with the excitation light for emission of fluorescent light, wherein an intensity distribution of the excitation light has at least one zero. The fluorescent light from the excited individual molecules of the substance is registered for different positions (XN) of the at least one zero of the intensity distribution of the excitation light in the region of interest in the sample. In this case, distances between closest adjacent positions (XN) of the at least one zero of the intensity distribution of the excitation light in which the fluorescent light from the excited individual molecules of the substance is registered are not greater than half the minimum value d. The locations (XM) of the individual molecules of the substance are then derived from the profile of the intensity (I) of the fluorescent light from the respective molecule against the positions (XN) of the at least one zero of the intensity distribution of the excitation light in the region of interest in the sample. |
