BIOSYNTHESIS OF CAFFEIC ACID AND CAFFEIC ACID DERIVATIVES BY RECOMBINANT MICROORGANISMS

摘要

Microorganisms are genetically engineered to synthesize caffeic acid from simple carbon sources via a tyrosine intermediate by means of a dual pathway that utilizes both endogenous and engineered enzymatic activities.

主权项

1. A method for producing caffeic acid comprising:
culturing a genetically engineered cell comprising p-coumarate 3-hydroxylase (C3H) enzyme activity, wherein said cell has been metabolically engineered to express or overexpress a tyrosine ammonia lyase (TAL) and to overexpress a 4-hydoxyphenylacetate 3-hydroxylase (4HPA3H) enzyme or enzyme complex, under conditions effective to produce caffeic acid; and isolating the caffeic acid from the cell or the culture medium.