发明名称 |
OPTIMIZED INSECT-BASED EX VIVO MODEL FOR TESTING BLOOD-BRAIN BARRIER PENETRATION AND METHOD FOR EXPOSING INSECT BRAIN TO CHEMICAL COMPOUNDS |
摘要 |
There is provided an ex-vivo insect screening model to determine blood-brain barrier penetration of different chemical compounds. The method comprises the steps: • Optionally anesthetizing the insect • Fixing the head of the insect • Dissecting out the brain of the insect head thereby removing the brain from its cuticle • Optinally removing the neural lamella of the brain • Treating the brain with a solution of the chemical compound • Washing anf homogenising or ultra sound disintegrating the brain • Determining the concentration of the chemical compound in the homogenised brain material and • Calculating the penetration of the chemical compound through the blood-brain barrier The concentration of the chemical compound is determined by LC/MS and the chemical compound can be a CNS drug. |
申请公布号 |
EP2795323(A4) |
申请公布日期 |
2015.07.01 |
申请号 |
EP20120859142 |
申请日期 |
2012.12.12 |
申请人 |
ENTOMOPHARM APS |
发明人 |
NIELSEN, PETER AADAL;ANDERSSON, GUNNAR;ANDERSSON, OLGA |
分类号 |
G01N33/50 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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