Method for determining derivatized analytes in a separated biological fluid

摘要

The present invention comprises methods for determining the presence, amount, or concentration of analytes of interest including Vitamin D and other secosteroids from biological samples, through derivatization, with improved speed and ease of analysis and improved sensitivity to mass spectrometry. In a preferred embodiment, the present invention comprises methods for determining the presence, amount, or concentration of analytes of interest, including Vitamin D and other secosteroids from whole human blood, through derivatization, and using a plasma collection device to facilitate collection, separation, and preparation of the sample for derivatization and analysis.

主权项

1. A method for determining the presence, concentration, or amount of analytes of interest in a sample comprising the steps of:
a. collecting a biological fluid; b. separating a liquid sample from said biological fluid; c. aliquoting said sample; d. derivatizing analytes of interest within said sample, wherein the derivatizing agent comprises at least one of: 4-(1-methyl-4-pyridinylmethyl)-1,2,4-triazolidine-3,5-dione, 4-phenyl-1,2,4-triazolidine-3,5-dione, and 4-ferrocenylmethyl-1,2,4-triazolidine-3,5-dione, and their isomers, isotopes, and analogs; e. transferring said sample to a preparation vessel; f. purifying said sample; and g. analyzing analytes of interest of said sample.