Highly sensitive biomarker panels

摘要

Cardiovascular disease, e.g., congestive heart failure, is often first diagnosed after the onset of clinical symptoms, eliminating potential for early intervention. The invention provides a multi-marker immunoassay, including cardiac pathology and vascular inflammation biomarkers, yielding a more sensitive assay for early detection of CHF in plasma. A panel consisting of cardiac pathology (cTnI, BNP) and vascular inflammation (IL-6, TNFα, IL-17a) biomarkers provided a sensitivity of 94% for association with CHF.

主权项

1. A method for detecting or monitoring a cardiovascular condition associated with cardiomyocyte damage in a subject, comprising detecting the concentration of two or more biomarkers in a sample from the subject, wherein the biomarkers comprise Troponin-I (cTnI) and Interleukin 17a (IL-17a), and wherein the limit of detection of at least one biomarker is less than about 20 pg/ml, comparing the concentration of the biomarkers to threshold concentrations for the biomarkers, and determining the presence of the cardiovascular condition when the concentration of the biomarkers in the sample is greater than the threshold concentrations.