发明名称 |
Method and system for calibrating a flow cytometer |
摘要 |
A method and system for calibrating a flow cytometer to a refractive index X, the method comprising: analyzing a first mixture of particles in the cytometer and recording the results produced, wherein the first mixture comprises particles having refractive index X, wherein the particles of refractive index X have a plurality of different sizes; analyzing a second mixture of particles in the cytometer and recording the results produced, wherein the second mixture comprises particles having refractive index Y, wherein refractive index Y does not equal refractive index X, and wherein the particles of refractive index Y have a plurality of different sizes, wherein there is at least a partial overlap between the particle sizes in the first and second mixtures, and wherein the size of at least one particle of the second mixture is known; and using the results produced by the first and second mixture to calibrate the cytometer to refractive index X. |
申请公布号 |
US9068915(B2) |
申请公布日期 |
2015.06.30 |
申请号 |
US201313779701 |
申请日期 |
2013.02.27 |
申请人 |
OJK Consulting Ltd. |
发明人 |
Kenyon Oliver |
分类号 |
G01N15/14;G01N31/00;G01N15/10 |
主分类号 |
G01N15/14 |
代理机构 |
Arent Fox LLP |
代理人 |
Arent Fox LLP |
主权项 |
1. A method of calibrating a flow cytometer to a refractive index X, the method comprising:
analyzing a first mixture of particles in the cytometer and recording the results produced, wherein the first mixture comprises particles having refractive index X, wherein the particles of refractive index X have a plurality of different sizes; analyzing a second mixture of particles in the cytometer and recording the results produced, wherein the second mixture comprises particles having refractive index Y, wherein refractive index Y does not equal refractive index X, and wherein the particles of refractive index Y have a plurality of different sizes, wherein there is at least a partial overlap between the particle sizes in the first and second mixtures, and wherein the size of at least one particle of the second mixture is known; and using the results produced by the first and second mixture to calibrate the cytometer to refractive index X. |
地址 |
Northwood, Middlesex GB |